Molecular and technological approaches to evaluate strain biodiversity in Hanseniaspora uvarum of wine origin

A. Capece, C. Fiore, A. Maraz and P. Romano
Journal of Applied Microbiology 2005, 98, 136–144

Aims: The characterization by molecular and physiological methods of wild apiculate strains, isolated from "Aglianico del Vulture" grape must.
Methods and Results: The restriction analysis of 18S rDNA allowed the identification of strains at the species level, which were predominantly Hanseniaspora uvarum. The RAPD analysis and the evaluation of technological traits, such as the metabolic and enzymatic activities, were useful to evaluate the polymorphism of this species.
Conclusions: The RAPD analysis clustered the wild H. uvarum strains in four main genetic groups and a very high phenotypic variability confirmed this genetic polymorphism. The technological variables, which determined the strain biodiversity differed significantly, demonstrating that these technological traits are strain dependent. A certain correlation was found between the strain behaviour and its isolation zone, indicating the influence of the environmnt on the genetic patrimony of the population.
Significance and Impact of the Study: Tehe genetic and technological biodiversity recorded among H. uvarum wild strains represents the basis for organizing a collection of apiculate strains exhibiting oenological characteristics at different levels, such as high/low production of secondary compounds, and, therefore, potentially useful for a selection programme.
Keywords: enzymatic and metabolic activities, Hanseniaspora uvarum, PCR-RFLP analysis, RAPD-PCR analysis, strain biodiversity.

Molecular and technological approaches to evaluate strain biodiversity in Hanseniaspora uvarum of wine origin